Studies on the Chemical Constitution of Agar-agar. XVIII. Isolation of a New Crystalline Disaccharide by Enzymatic Hydrolysis of Agar-agar

The Introduction of Agar-agar into Bacteriology.

Among fundamental developments in bacteriology the elaboration of solid culture substrates will always hold first place. With the introduction of a method for isolating micro6rganisms and growing them in pure culture, bacteriologists possessed the means for studying what Ferdinand Cohn (1872) called "Die kleinsten lebenden Wesen." Credit for the discovery of the solid medium technique is common...

Differentiation of Candida dubliniensis on chrom agar and Pal's agar.

Difference in expression of putative virulence factors and in antifungal susceptibility among different Candida species has raised the need for species-level identification. The close relationship of Candida dubliniensis with C. albicans has led to misidentification of C. dubliniensis isolates as C. albicans. Phenotypic tests include ability to produce chlamydospore on casein agar, colony colou...

Studies of Agar-digesting Bacteria.

Blood agar, chocolate agar, and Mycobacterium tuberculosis.

We have read with interest the recent paper by Drancourt et al. in which they reviewed the use of blood agar medium for the primary isolation of Mycobacterium tuberculosis (2). They state that to their knowledge “no comparative study comparing the efficacy of blood-based agars and egg-based agars has been carried out, and even the ability of blood agar to support growth of M. tuberculosis was f...

Isolation of Shigellae. 8. Comparison of xylose lysine deoxycholate agar, hektoen enteric agar, Salmonella-Shigella agar, and eosin methylene blue agar with stool specimens.

Two enrichment broths and four plating media were compared for efficiency of detection of enteric pathogens from 1,597 stool specimens. Of 170 salmonellae isolated from the composite of all methods, direct streaking yielded but 54%, whereas enrichment in gram-negative broth found 87% and Selenite-F broth 97%. By contrast, gram-negative broth produced 100% of the 17 shigellae, Selenite-F broth b...